Extended Data Fig. 9: Comparison of track scores in different conditions.
a, Consistency of Tracking-seq between replicates. Batch1 and batch2 were two biological replicates while batch2_1 and batch2_2 were two technical replicates. The condition is 500, 000 HEK293T cells with sgRNA HEK293_site_4 after 96-hour Cas9 editing. Each dot indicates a candidate site. b, Comparison of track scores between PE2 and PE3 using VEGFA_site_2 in HEK293T cells. c, Comparison of track scores between 150, 000 cells and 60, 000 cells of mHSPCs with Cas9 editing using Pcsk9-gP+G. d, Comparison of track scores among mHSPCs, mESCs, NIH3T3 cells, and AML12 cells with Cas9 editing using Pcsk9-gP+G. For mHSPCs, the mean track score of the two experiments (150, 000 cells and 60, 000 cells) was used. For mESCs, one experiment of 30,000 cells was conducted. For NIH3T3, the mean track score of the two experiments (100, 000 cells and 200, 000 cells) was used. For AML12, one experiment of 100,000 cells was conducted. The orange dots and red dots represent the tested differential off-target sites (the same as Fig. 6a).
