Extended Data Fig. 2: Dynamics of Tracking-seq signal over time for HEK293T with sgRNA HEK293_site_4.
a, The signal of the on-target site and an off-target site were shown. The condition is 500, 000 HEK293T cells with sgRNA HEK293_site_4. b, The dynamics of Tracking-seq signals over time (n = 1 for each condition). The time points indicate the periods after the PEI transfection of HEK293T cells. Background indicates the mean score of all negative sites (see Methods for more details). c, The expression kinetics of editing proteins (left) and guide RNAs (right) measured by the fluorescent intensity at different time points after transfrection. (n = 1 for each condition).
