Fig. 5: ACE characterizes T cell signaling network dynamics modulated by POF sample cocultures.
From: Signal amplification by cyclic extension enables high-sensitivity single-cell mass cytometry
a, Proliferation and TCR signaling effects of POF on human T lymphocytes were assessed using dye dilution and flow cytometry, and through the ACE-based 30-plex TCR signaling mass cytometry analysis, respectively. b, Percentage of proliferative T lymphocytes after treatment with anti-CD3/anti-CD28 beads or with indicated POF samples. Data are presented as mean values ± s.d.; n = 3 individual experiments. ***P < 0.001 using a two-tailed t-test); ***P value = 0.0000832. c, TCR stimulation was performed in the presence of POF or medium control. The cells were fixed 0, 2, 5, 10, 15 and 30 min later, and analyzed by ACE. The timepoint when the maximal signal for each specific phosphorylation site was observed after TCR stimulation is shown. d, The integrated signal corresponding to each phosphorylation site across the stimulation timecourse was calculated as the AUC/HMP. This differs across all POF treatment conditions and the medium-only control as indicated by circle size and color. e, Signaling trajectories for p-CD3ζ, p-ZAP70, p-SLP76, p-BTK/ITK, p-ERK1/2 and p-S6 for each POF sample and the medium-only control are shown. Dashed lines indicate HMP signals; shading defines the AUC/HMP.
