Extended Data Fig. 9: Sorting of SARS-CoV-2 S1-specific human ASCs.
(a) Comparison of IgG signal originating from ASCs in BG-agarose beads incubated with (+VHH) or without VHH–SNAP (−VHH). The plots show 823 (−VHH) and 6,870 (+VHH) events at 2% contour level. (b) Representative gating strategy for identifying S1/RBD-specific human ASCs. (c) Neutralization of SARS-CoV-2 by hS1-1. Wild-type SARS-CoV-2 (MOI = 0.01) was pre-incubated with a 3-fold dilution series of hS1-1 and then used to infect luminescent reporter cells. Levels of infection after 24 h were quantified as % of maximum luminescence. Mean values of two technical replicates are shown, representative of two independent experiments. IC50, half-maximal inhibitory concentration.
