Extended Data Fig. 1: Synthesis and characterization of benzylguanine (BG)-agarose. | Nature Biotechnology

Extended Data Fig. 1: Synthesis and characterization of benzylguanine (BG)-agarose.

From: Rapid discovery of monoclonal antibodies by microfluidics-enabled FACS of single pathogen-specific antibody-secreting cells

Extended Data Fig. 1

(a) Synthesis of BG-agarose. (b) Assessment of 1.5% (wt/vol) BG-agarose capture valency. BG-agarose beads were incubated with different amounts of GFP-SNAP, and the GFP fluorescence of the beads was analyzed by flow cytometry. The fluorescence signal saturates between 1.3 and 2.7 × 109 GFP molecules/bead. Data are represented as mean values ± SD, and at least 18,827 beads were analyzed per condition (unpaired t-test, two-sided p < 0.0001). Data from one independent experiment is shown.

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