Extended Data Fig. 5: Workflow with spleen-derived ASCs. | Nature Biotechnology

Extended Data Fig. 5: Workflow with spleen-derived ASCs.

From: Rapid discovery of monoclonal antibodies by microfluidics-enabled FACS of single pathogen-specific antibody-secreting cells

Extended Data Fig. 5

(a) Mouse immunization and analysis scheme. (b) Comparison of IgG signal originating from ASCs in BG-agarose beads incubated with (+VHH) or without VHH–SNAP–FLAG (−VHH; different experiment than shown in c). The plots show 3,663 (−VHH) and 2,290 (+VHH) events at 2% contour level. (c) Representative gating strategy for identifying OVA-specific ASCs from spleen. Live ASCs inside hydrogel beads were gated as live/FLAG+/CD138+ (VHH–SNAP contains a FLAG tag).

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