Fig. 1: Optimized DISCO clearing for imaging nanocarriers at low doses.
From: Nanocarrier imaging at single-cell resolution across entire mouse bodies with deep learning
a, Scheme of SCP-Nano—a pipeline for mapping and quantifying the biodistribution of any fluorescently labeled nanocarrier throughout the entire mouse body with single-cell resolution and high sensitivity. b,c, Bioluminescence imaging (ventral) 6 h after intravenous injection of 0.5 mg kg−1 (b) and 0.0005 mg kg−1 (c) of luciferase mRNA-carrying LNPs. d–f, Whole body light sheet imaging of mice intravenously injected with 0.0005 mg kg−1 Alexa Fluor 647–labeled EGFP mRNA-carrying LNPs and cleared with our refined DISCO clearing methods. This approach enables the visualization of mRNA delivery throughout the entire mouse body, including the liver (e) and spleen (f), at cellular resolution. g–m, Visualization of whole mouse body LNPs after intranasal delivery of 0.0005 mg kg−1: maximum intensity projection (g) and single optical slice views (h–l); representative individual optical slices of the lung (m).
