Fig. 5: SCP-Nano reveals targeted delivery of DNA origami.
From: Nanocarrier imaging at single-cell resolution across entire mouse bodies with deep learning
a–f, Cell-level resolution biodistribution of non-targeted and CX3CR1 antibody immune-cell-targeted (illustrated on the left) origami throughout the entire mouse body (c,d) and in detailed views of the liver (e,f; illustrating single-cell resolution) 20 min after intravenous injection of 50.725 mg kg−1 DNA origami. g, Intra-tissue distribution of the immune cell and non-targeting origami (red) and co-staining with target cell marker CX3CR1 (left, yellow) and general immune cell marker CD68 (right, yellow) in confocal images of individual liver slices. h, Quantification of the co-localization of untargeted and CX3CR1-targeted origamis with CX3CR1+ and CD68+ cells (n = 3, mean ± s.d.). i, The prediction accuracy of DNA origami detection by SCP-Nano algorithm in different organs. j, Density map of CX3CR1 immune-cell-targeted origami distribution throughout the entire mouse body. k, SCP-Nano-based quantification of the biodistribution of CX3CR1 immune-cell-targeted origami in different organs.
