Extended Data Fig. 6: Efficient low-MOI editing can be achieved with high density transductions.
From: SEED-Selection enables high-efficiency enrichment of primary T cells edited at multiple loci
a, Comparison of high and low MOI editing protocols. b–d, T cells were edited with RNPs targeting TRAC and B2M ( + M3814) and transduced with TRAC-CAR and B2M-CD47 SEED HDRTs in a G-REX plate at an MOI of 3 × 104 (per AAV). Edited cells were then immunomagnetically purified with anti-TCR and anti-B2M (n = 3 donors). b, Representative flow cytometry plots of TCR, CAR, B2M, and CD47 expression. c, Percentage of B2M+ or TCR+ cells and fully edited cells (TCR– CAR + B2M– CD47 + ). d, Number of cells generated from editing 4 × 106 cells followed by a 10-day expansion.
