Extended Data Fig. 1: The association between relative error by short read-based tools and K-value.

Boxplot shows the median RE of genes within each K-value group across sequencing depths (n = 9) quantified using five different short read-based tools. Only genes with expression levels TPM > 1 ware retained for visualization. RE: relative error. In box plots, the hinges represent the first and third quartiles, the center line represents the median, and the whiskers extend to the smallest and largest data points within 1.5 interquartile from the hinges. All box plots in the subsequence analysis have the same definition unless specified.