Extended Data Fig. 7: Structural analysis of OrufIscB-REC-swap 49-ωRNA-target DNA complex, related to Fig. 2.
From: Evolution-guided protein design of IscB for persistent epigenome editing in vivo
(A) Flow chart of cryoEM data analysis of OrufIscB-REC-swap 49-ωRNA-target DNA complex including example micrographs of the imaged particles (top row), estimated particle clusters (second row). (B) Representative cryo-EM image from 7,580 movies (top) and representative 2D averages (bottom) of the OrufIscB-REC-swap 49-ωRNA-target DNA complex. (C) Cryo-EM density of the OrufIscB-REC-swap 49-ωRNA-target DNA complex. Light blue: OrufIscB-REC-swap 49; green, inserted REC domain; purple: ωRNA; pink, DNA non-target strand; light pink, DNA target strand. (D) EM density and atomic model of the inserted REC domain, HNH domain, the catalytic site of the RuvC domain and the catalytic site of the HNH domain.
