Extended Data Fig. 1: Single cell feature selection demonstrated using the data shown in Fig. 1b-d. | Nature Biotechnology

Extended Data Fig. 1: Single cell feature selection demonstrated using the data shown in Fig. 1b-d.

From: Proteoform profiling of endogenous single cells from rat hippocampus at scale

Extended Data Fig. 1

From 752 optically-registered cells, 529 single cells were detected based on filtering of distance and scPiMS sampling conditions (annotated in panel (a)). Location of the 529 single cells are registered in the pixel-based graph in panel (a). (b) Cellogram single cell feature selection purely based on peak features (more in Methods). Using a peak threshold of 750 ions and a spacing of 7 scans, 529 single cell-like peak profiles were registered, which was well correlated with the optical registration and statistical modeling.

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