Chemical proteomics has brought rigor to covalent drug discovery and drugs to the clinic. Can it deliver a new generation of drug targets?
Enjoying our latest content?
Log in or create an account to continue
- Access the most recent journalism from Nature's award-winning team
- Explore the latest features & opinion covering groundbreaking research
or
Change history
07 July 2025
Since the version of the article initially published, Jaimeen Majmudar’s title was amended to “Associate Research Fellow” in the HTML and PDF versions of the article.
References
Liu, Y., Patricelli, M. P. & Cravatt, B. F. Proc. Natl Acad. Sci. USA https://doi.org/10.1073/pnas.96.26.14694 (1999).
Greenbaum, D. et al. Mol. Cell. Proteomics https://doi.org/10.1074/mcp.t100003-mcp200 (2002).
Weerapana, E. et al. Nature https://doi.org/10.1038/nature09472 (2010).
Janes, M. R. et al. Cell https://doi.org/10.1016/j.cell.2018.01.006 (2018).
Baltgalvis, K. A. et al. Nature https://doi.org/10.1038/s41586-024-07318-y (2024).
Klebba, J.E. et al. Preprint at bioRxiv https://doi.org/10.1101/2024.12.17.629001 (2024).
Kavanagh, M. E. et al. Nat. Chem. Biol. https://doi.org/10.1038/s41589-022-01098-0 (2022).
Vinogradova, E. V. et al. Cell https://doi.org/10.1016/j.cell.2020.07.001 (2020).
Kuljanin, M. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-020-00778-3 (2021).
Parker, C. G. et al. Cell https://doi.org/10.1016/j.cell.2016.12.029 (2017).
Wobst, H. J. et al. JCI Insight https://doi.org/10.1172/jci.insight.182876 (2024).
Bachovchin, D. A., Brown, S. J., Rosen, H. & Cravatt, B. F. Nat. Biotechnol. https://doi.org/10.1038/nbt.1531 (2009).
Johnson, D. S. et al. ACS Med. Chem. Lett. https://doi.org/10.1021/ml100190t (2011).
Rocha, J.-F. et al. Clin. Pharmacol. Ther. https://doi.org/10.1002/cpt.2290 (2022).
Van Esbroeck, A. C. M. et al. Science https://doi.org/10.1126/science.aaf7497 (2017).
Huang, Z. et al. ACS Chem. Biol. https://doi.org/10.1021/acschembio.8b01097 (2019).
D’Souza, D. C. et al. Lancet Psychiatry https://doi.org/10.1016/S2215-0366(18)30427-9 (2018).
Wang, Y. et al. Nat. Chem. https://doi.org/10.1038/s41557-019-0351-5 (2019).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Garber, K. Probing the proteome. Nat Biotechnol 43, 1216–1220 (2025). https://doi.org/10.1038/s41587-025-02737-2
Published:
Issue date:
DOI: https://doi.org/10.1038/s41587-025-02737-2