Extended Data Fig. 1: Structure and conservation of the cspG RNA thermometer. | Nature Biotechnology

Extended Data Fig. 1: Structure and conservation of the cspG RNA thermometer.

From: Identification of conserved RNA regulatory switches in living cells using RNA secondary structure ensemble mapping and covariation analysis

Extended Data Fig. 1

(a) Secondary structure models of the 5′ UTR of cspG at 37 °C and 10 °C, with overlaid in vitro DMS reactivities, along with reactivity profiles and base-pairing probabilities for both conformations. Reactivities are averaged across two independent experiments. (b) Structure models for the two conformations of the identified cspG RNA thermometer, inferred by phylogenetic analysis. Base-pairs showing significant covariation (as determined by R-scape) are boxed in dark green (E-value < 0.05). Helices showing helix-level covariation support (E-value < 0.05) are boxed in light green.

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