Extended Data Fig. 4: Examples of edited, mixed, and unedited genotypes for sequence replacement with DarT2DLAA-nScCas9 targeting kanR* in E. coli. | Nature Biotechnology

Extended Data Fig. 4: Examples of edited, mixed, and unedited genotypes for sequence replacement with DarT2DLAA-nScCas9 targeting kanR* in E. coli.

From: Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes

Extended Data Fig. 4

Representative Sanger sequencing results from E. coli colonies transformed with a plasmid that contained a repair template to mediate the replacement of 91 bp at the kanR* site; 45 bp upstream and 45 bp downstream (+45/-45) of the ADP-ribosylated thymine.

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