Extended Data Fig. 5: ADPr-TAE with DarT2DLAA-nScCas9 at different sites in E. coli MG1655 and at two sites in the flgG gene in S. enterica LT2. | Nature Biotechnology

Extended Data Fig. 5: ADPr-TAE with DarT2DLAA-nScCas9 at different sites in E. coli MG1655 and at two sites in the flgG gene in S. enterica LT2.

From: Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes

Extended Data Fig. 5

a, ADPr-TA editing through substitution at the aaaD, punR, ygcQ, and yheO genes of E. coli MG1655. All substitutions introduced silent mutations. b, ADPr-TA editing through substitution (flgG site 1) or deletion (flgG site 2) at the flgG gene of S. enterica LT2. All experiments for E. coli and S. enterica were performed in duplicate, and eight colonies were screened through colony PCR and Sanger sequencing per duplicate experiment.

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