Extended Data Fig. 10: Assessing sgRNA-independent off-target editing via DNA ADP-ribosylation.
a, General configuration for assessing sgRNA-independent off-targeting through the generation of an orthogonal R-loop via dSaCas9. b, On-target editing activities under targeting conditions. c, Base mutation across dSaCas9 target sites under targeting and non-targeting conditions by the editor. No editor: expression of the targeting sgRNA without an editor. Bars and error bars in b and c represent the mean and s.e.m. of three independent replicates without selection or sorting.
