Extended Data Fig. 1: ADP-ribosylation of a 5′-TCTC-3′ motif on ssDNA by cell-free expressed EPEC DarT2 blocks DNA polymerisation by E. coli Klenow fragment. | Nature Biotechnology

Extended Data Fig. 1: ADP-ribosylation of a 5′-TCTC-3′ motif on ssDNA by cell-free expressed EPEC DarT2 blocks DNA polymerisation by E. coli Klenow fragment.

From: Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes

Extended Data Fig. 1

ssDNA oligos harboring a 5′-TCTC-3′ (a) or 5′-ACTC-3′ (b) motif were treated with wildtype or inactivated (dDarT) EPEC DarT2, annealed with a 3′-complementary Fam-tagged oligo, and treated with E. coli DNA Polymerase I (Klenow Fragment). Primer extension products were run on denaturing polyacrylamide gels and imaged under UV light before (left, only showing elongated Fam-tagged primer) and after (right, imaging all DNA) SYBR Gold staining. Images are representative of triplicate independent experiments. dDarT2: EPEC DarT2 with the inactivating E170A mutation.

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