Extended Data Fig. 2: Representative Sanger sequencing results after targeting the kanR* site with DarT2D-nScCas9 in E. coli in the presence of a repair template. | Nature Biotechnology

Extended Data Fig. 2: Representative Sanger sequencing results after targeting the kanR* site with DarT2D-nScCas9 in E. coli in the presence of a repair template.

From: Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes

Extended Data Fig. 2

The target thymine in the DarT2 motif is labelled in red. The mutated bases are highlighted in red. Results are shown for a screened kanamycin-resistant colony under targeting conditions (top) and a colony under non-targeting conditions (bottom).

Back to article page