Fig. 1: Capturing complete genome sequences from a complex soil metagenome.
From: Bioactive molecules unearthed by terabase-scale long-read sequencing of a soil metagenome
a, The limitations of short-read technology restrict complete genomic sequencing to cultured isolates; emerging long-read sequencing may overcome this challenge. b, Nanopore sequencing read-length distribution comparison of soil metagenomic DNA prepared by direct hot CTAB extraction (old) and the newly developed and optimized nycodenz method (new). c, Assembly summary of high-quality nanopore soil metagenomic sequencing at metaFlye’s input limit. d, Assembly length plot of >1 Mbp metaFlye generated contigs with an estimation of genome completeness based on the presence of all rRNA genes (5S, 16S and 23S) and at least 18 unique tRNAs. Plots are separated into circular and linear assemblies.
