Fig. 4: MOA studies for trigintamicin and erutacidin.
From: Bioactive molecules unearthed by terabase-scale long-read sequencing of a soil metagenome
a, Trigintamicin structure. b, ITC (top) and isotherm curves (bottom) of S. aureus ClpX against trigintamicin and its biologically inactive R-isomer. Data are representative of at least two independent determinations. c, Erutacidin structure. d, Resistance changes of S. aureus and A. baumannii during serial passaging in the presence of erutacidin or apramycin. e, S. aureus kill curve elicited by erutacidin at varied concentrations. f, DiSC3(5) depolarization assay of S. aureus in the presence of antibiotics at 2× MIC or DMSO. g, Fold change in erutacidin MIC against S. aureus caused by exogenous supplementation of various lipids. LOD, limit of detection; CL, cardiolipin; LPE, lysophosphatidylethanolamine; SH, sphingomyelin; AS, N-acetylsphingosine; MK, menaquinone-4. Data in d–g are presented as the mean values ± s.d. (n = 3 biologically independent replicates).
