Extended Data Fig. 6: Ruminant haSCs harbour potency for the formation of embryoid bodies and teratoma.
From: Generation of modified cows and sheep from spermatid-like haploid embryonic stem cells
a. Morphology of embryoid bodies (EBs) derived from ruminant haSCs. n = 3 independent experiments with similar results. Scale bar, 100 μm. b. RT-PCR analysis of the expression of three germ-line markers in EBs derived from ruminant haSCs. n = 3 independent experiments with similar results. c. Further immunostaining for three germ-line markers in EBs derived from ruminant haSCs, including ectoderm (NESTIN), mesoderm (BRACHYURY), and endoderm (GATA4). Scale bar, 100 μm. d. FACS analysis of haploid cells in EBs derived from ruminant haSCs at the indicated time points. e. Teratoma produced by injection of ruminant haSCs into the immunodeficient mice. f. Morphology of teratoma produced by ruminant haSCs. g. FACS analysis of haploid cells in teratoma derived from ruminant haSCs at the indicated time points. h. Summary of teratoma formation assay by using ruminant haSCs.
