Extended Data Fig. 3: FACE medium supports ruminant haSCs long-term culture.
From: Generation of modified cows and sheep from spermatid-like haploid embryonic stem cells
a. List of different combinations of small molecules used in this study. b. Summary of b-haSCs derivation efficiency from bovine haploid androgenetic ICMs by different combinations of small molecules. c. Morphology of bovine haploid androgenetic ICMs under different combinations of small molecules. Scale bars, 200 μm. d. The total cell numbers of b-haSCs during cell passaging under different concentrations of Activin-A. Cells were plated at 5 ×105 cells per well and were cultured for 4 days; Data are mean ± s.d. (n = 3 independent experiments); P values are from unpaired, two-tailed Student’s t-tests. e. Haploidy analysis in b-haSCs derived using 10 ng/mL and 20 ng/mL of Activin-A. The figure on the left is the same as Fig. 1e; n = 3 independent experiments with similar results. Note that 10 ng/mL Activin-A was beneficial for maintaining haploidy stability.
