Supplementary Figure 9: Properties of topologically associating domains.

a, The plot depicts the cumulative distribution function of the mean CDTS values (in ≤10-kb windows) inside and outside TADs. TAD and non-TAD regions were divided into 10-kb windows (the overhang windows were discarded if smaller than 1 kb). The most constrained TAD windows are those identified by Hi-C as present in five or more cell types. TAD in at least one cell type versus no TAD: Kolmogorov–Smirnov two-sided test, P = 2.2 × 10^–16. The total number of windows per group was as follows: non-TAD (n = 19,999 covering 139 Mb), TAD ≥1 cell type (n = 331,471 covering 2.4 Gb), TAD ≥2 cell types (n = 134,486 covering 911 Mb), TAD ≥5 cell types (n = 4,558 covering 29 Mb). b, The plot depicts the cumulative distribution function of the mean CDTS values (in ≤10-kb windows) of anchor and loop regions within TADs. Anchor and loop regions were divided into 10-kb windows (the overhang windows were discarded if smaller than 1 kb). The anchor regions are consistently more constrained than the loops within the same TADs. Anchor in at least one cell type versus loop in at least one cell type: Kolmogorov–Smirnov two-sided test, P = 2.7 × 10^–14. The total number of windows per group is as follows: anchor ≥1 cell type (n = 2,954 covering 17 Mb), anchor ≥2 cell types (n = 1,321 covering 7 Mb), anchor ≥5 cell types (n = 38 covering 0.2 Mb), loop ≥1 cell type (n = 271,356 covering 1.8 Gb), loop ≥2 cell types (n = 117,581 covering 753 Mb), loop ≥5 cell types (n = 4,020 covering 24 Mb). TAD, topologically associating domain.