Supplementary Figure 4: AGO2 and DICER expression in NUDT21 KD HeLa cell.
From: 3′ UTR shortening represses tumor-suppressor genes in trans by disrupting ceRNA crosstalk
Detection of AGO2 associated genes in HeLa cells by real time PCR (a) RNA-binding protein immunoprecipitation (RIP) was performed with AGO2 antibody, normal mouse IgG was served as a control. The RIP complex was detected by western blot with another AGO2 antibody from Rat. (b) AGO2 associated genes were measured by real time PCR. Two candidate ceRNAs, PHF6 and LARP1, and LAMC1 3′UTR shortening gene were enriched up to 229.5, 343.6 and 232.1 fold. FOS was served as a positive control and 7SK snRNA as a negative control. (c) Dicer1 expression level in Ctrl and NUDT21 KD HeLa cells (d) Scatterplot showing miRNA expression levels in Ctrl or NUDT21 KD HeLa cells, where black lines indicate differentially expressed miRNAs (2-fold up or down).
