Supplementary Figure 7: Colony formation efficiency in MCF7 cells using two independent siRNAs. | Nature Genetics

Supplementary Figure 7: Colony formation efficiency in MCF7 cells using two independent siRNAs.

From: A transcriptome-wide association study of 229,000 women identifies new candidate susceptibility genes for breast cancer

Supplementary Figure 7

MCF7 cells were transfected with the indicated siRNAs and then reseeded after 16 h for colony formation (CF) assays. At day 14, colonies were fixed with methanol, stained with crystal violet, scanned and batch analyzed by ImageJ. Corrected CF efficiency (CFE) % = 100 ± (relative CFE in indicated siRNA – CFE in control siRNA (consi))/knockdown efficiency. Error bars, s.d. (n = 4). P values were determined by one-way ANOVA followed by Dunnett’s multiple-comparisons test: *P < 0.05. Related to Fig. 2b.

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