Supplementary Figure 10: C1D-EXOSC10 interaction anchors the exosome to the nucleolus
From: MiCEE is a ncRNA-protein complex that mediates epigenetic silencing and nucleolar organization
a, MLg cells were transfected with a control (Ctrl) or C1d-specific shRNA construct. Transfected cell were analyzed by confocal microscopy after double immunostaining using EXOSC10- and FBL-specific antibodies. DAPI, nuclear staining. Scale bar, 10 µm. The arrow delineates the position of the staining intensity profiles represented in the plots (right). See also Figures S6g. b, MLg cells were transfected with EXOSC10 full length (fl) or deletion mutant 1 (del1). Transfected cells were analyzed as in (a). Scale bar, 20 µm. c, ChIP analysis of Mafg- and Chmp2b-promoters using the indicated antibodies in MLE-12 cells transfected with Mirctrl, Mirlet7d or/and C1d-specific shRNA constructs. See Figure S6g. In a-b, representative results from 3 independent experiments. In all plots, data are shown as means ± s.e.m (n=3 independent experiments); asterisks, P values after one–way ANOVA, ***P˂0.001; **P˂0.01; *P˂0.05; ns, non-significant. The statistical test values of each plot are shown in the Supplementary Data Set 4
