Supplementary Figure 13: Distribution of PNH associated domains (PNHADs)
From: MiCEE is a ncRNA-protein complex that mediates epigenetic silencing and nucleolar organization
a, Distribution of normalized mapped reads from Mirlet7d-ChIRP-seq respective to the gene body and a ± 2 kb region to transcription start (TSS) and termination (TTS) sites. Norm tags, tag density normalized to input and represented as log2 values. Genes used for the analysis are the Mirletd7 targets identified in our RNA-seq after Mirlet7d-Pd shown in Fig. 1f. b, Aggregate plot for Mirctrl-, Mirlet7d-ChIRP-seq, H3K27me3-, UBF-ChIP-seq and input peaks in a ± 2 kb region respective to Mirlet7d peaks. Norm tags, mapped reads normalized to 10 million using annotatePeaks.pl from Homer. c, UCSC genome browser visualization of chromosomes (left) containing specific loci (right and bottom) enriched after Mirlet7d-ChIRP, UBF- and H3K27me3-ChIP. We hypothesized that the Mirlet7d-UBF-H3K27me3-common loci were PNH associated domains (PNHADs). Images represent indicated loci; black boxes, exons; lines, introns; arrow, transcription direction. Sites enriched with Mirlet7d, UBF and H3K27me3 (common sites) are represented as black boxes at the bottom of each loci. See Fig. 7d-e and S11d-f
