Supplementary Fig. 6: Distribution of neuronal cells following an assay of neuronal migration.

Migration assay from human iPSC-derived neural progenitor cells. The distance neuronal cell bodies migrate from the edge of a neuronal aggregate after 48 h is measured and binned into 30-μm increments, plotted as the average ± s.e.m. The graph of MDS, 1263A, and CTNNA2^KO stem cell–derived neurons shows a left-shifted pattern, as neurons from patients with Miller–Dieker syndrome or CTNNA2 mutations do not migrate as far as control neurons. Treatment with lentivirus encoding full-length CTNNA2 or the actin-binding domain (ABD) of αN-catenin (CTNNA2^ABD), but not αN-catenin lacking the ABD (CTNNA2^ΔABD), was sufficient to restore the distribution of neurons in the assay. Results shown from three different iPSC clones per patient or three CTNNA2^KO clones, 1,360 cells scored. The data are also displayed as a whisker box plot in Fig. 2a,c.