Supplementary Fig. 8: Expression of human CTNNA2 in control and CTNNA2-deficient NPCs.

a, Long and short exposures of a western blot for αN-catenin in control, control + CTNNA2, 1263A + CTNNA2, and CTNNA2^KO + CTNNA2 NPCs show similar levels of forced CTNNA2-GFP expression in the lentivirus-transduced NPC lines. A cleavage project was observed but not further investigated. Representative image shown, repeated in three independently generated patient-derived iPSC or CTNNA2^KO hESC clones. b, Long and short exposures of a western blot for αN-catenin in control, control + CTNNA2^ΔABD, 1263A + CTNNA2^ΔABD, and CTNNA2^KO + CTNNA2^ΔABD NPCs showed nearly endogenous levels of forced CTNNA2^ΔABD-GFP expression in lentivirus-transduced NPC lines. GAPDH loading control. Representative image shown, repeated in three independently generated patient-derived iPSC or CTNNA2^KO hESC clones. c. Long and short exposure of Western blot for GFP in Control, Control + CTNNA2^ABD, 1263A + CTNNA2^ABD, and CTNNA2^KO + CTNNA2^ABD NPCs show forced CTNNA2^ABD-GFP expression in lentivirus transduced NPC lines. GAPDH loading control. Representative image shown, repeated in three independently generated patient-derived iPSC or CTNNA2^KO hESC clones.