Supplementary Fig. 9: Loss of CTNNA2 does not disrupt neuroepithelial polarity in neural rosettes. | Nature Genetics

Supplementary Fig. 9: Loss of CTNNA2 does not disrupt neuroepithelial polarity in neural rosettes.

From: Biallelic loss of human CTNNA2, encoding αN-catenin, leads to ARP2/3 complex overactivity and disordered cortical neuronal migration

Supplementary Fig. 9

a, Immunofluorescence for αN-catenin in patient-derived neural rosettes. DAPI counterstain shows the cell nucleus. Patient rosettes showed absent αN-catenin staining (bottom). The white dashed line outlines the apical lumen. Representative image shown, repeated in three independently generated patient-derived iPSC clones. Scale bar, 25 μm. b, Immunofluorescence for apically localized markers in patient-derived neural rosettes. Shown is the primary cilia marker ARL13B in combinations with the tight junction marker ZO-1 and the adherens junction markers N-cadherin and β-catenin. DAPI counterstain shows the cell nucleus. Both control and patient-derived rosettes form polarized domains that are positive for apical markers and localize the primary cilium to this domain. Representative image shown, repeated in three independently generated patient-derived iPSC clones. Scale bar, 20 μm.

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