Supplementary Fig. 2: Expression of CTNNA2 encoding αN-catenin in developing human cortex and codistribution with markers of migration. | Nature Genetics

Supplementary Fig. 2: Expression of CTNNA2 encoding αN-catenin in developing human cortex and codistribution with markers of migration.

From: Biallelic loss of human CTNNA2, encoding αN-catenin, leads to ARP2/3 complex overactivity and disordered cortical neuronal migration

Supplementary Fig. 2

a, qRT–PCR analysis of CTNNA2 abundance across human tissues. The strongest expression is in fetal and mature brain, with lower levels in other tissues. GAPDH was used as a loading control. Quantitative expression of CTNNA2 was normalized to GAPDH and then to average CTNNA2 expression across all tissues. Experiments were repeated in triplicate, quantified at the right and plotted as the mean (bar) with each data point (red dot). Error bars, s.e.m. b, Location of αN-catenin relative to Nestin and codistribution with markers of neuronal migration including Dcx and Tuj1 (arrows) at embryonic day (E) 13.5 in mouse. CP, cortical plate; SVZ, subventricular zone; VZ, ventricular zone; DAPI (blue). Repeated in three biological replicates. Scale bar, 50 μm. c, Localization of αN-catenin (brown) in human 20-week gestation fetal cortex in CP and marginal zone (MZ), compared with TUJ1 and DCX, counterstained with Nissl. Bottom, αN-catenin (brown) shows signal along the apical surface of the VZ, similar to Nestin. Repeated in duplicate. Scale bar, 100 μm.

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