Supplementary Fig. 4: CTNNA2-mutant NPCs have less αN-catenin. | Nature Genetics

Supplementary Fig. 4: CTNNA2-mutant NPCs have less αN-catenin.

From: Biallelic loss of human CTNNA2, encoding αN-catenin, leads to ARP2/3 complex overactivity and disordered cortical neuronal migration

Supplementary Fig. 4

Western blot of αN-catenin in control, 1263 affected (A), and CTNNA2KO NPCs show the patient, and CRISPR–SpCas9-mediated mutations reduce the levels of αN-catenin protein. GAPDH was a loading control. Densitometry quantification (at right) is shown from three independent iPSC clones per patient and three CTNNA2KO clones. Top box, Q3; bottom box, Q1; median displayed. The whiskers represent the minimum and maximum values observed in the dataset. **, significant P values (see “Statistics and reproducibility”).

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