Supplementary Figure 2: Quantification of neutral comet assay results after metabolite treatment of cell lines. | Nature Genetics

Supplementary Figure 2: Quantification of neutral comet assay results after metabolite treatment of cell lines.

From: Krebs-cycle-deficient hereditary cancer syndromes are defined by defects in homologous-recombination DNA repair

Supplementary Figure 2

(a-c) Quantification of neutral comet assay performed in YUNK1 cells 24 h after the addition of indicated doses of (a) monoethyl-fumarate, (b) monoethyl-succinate and (c) dimethyl-succinate to the cell culture medium. (d) Quantification of neutral comet assay performed in HeLa cells 24 h after addition of the indicated doses of succinate to the cells. (e-h) Quantification of neutral comet assay performed in (e) HeLa, (f) HEK293FT, (g) 786-O, and (h) RCC4 cells 24 h after the addition of indicated concentrations of metabolites. (i-j) Quantification of immunofluorescent γH2AX foci in YUNK1 cells 24 h after the addition of the indicated doses of (i) dimethyl-fumarate and (j) succinate. (k-l) Quantification of immunofluorescent p53BP1 foci in YUNK1 cells 24 h after the addition of the indicated doses of (k) dimethyl-fumarate and (l) succinate. For all panels n = 3, bars represent mean + /-SEM and statistical analysis was by two-sided t-test and df = 4.

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