Supplementary Figure 5: Effective knockdown of DNA repair factors using siRNA.
From: CRISPR–Cas9 genome editing in human cells occurs via the Fanconi anemia pathway
a, Knockdown and editing of DSB repair factors reveals genetic differences between HDR and SSTR. K562 cells expressing a BFP reporter were treated with the indicated siRNAs and edited by co-administration of RNP targeting the BFP reporter with ssODN or dsDonor containing a BFP-to-GFP mutation. Data presented are the mean ± s.d. of n = 2 independent experiments. b, siRNA of RAD51, PARP1, and LIG4 in the K562 cells from a. Cells were siRNA treated for 48 h prior to editing. Fold depletion of the siRNA-target transcript over controls (ACTB, GAPDH) was measured by qPCR. Data presented represent the transcriptional state of the cells at the time of editing. The mean ± s.d. was calculated from n = 2 independent experiments. c, siRNA knockdown of FANCA and FANCF in human dermal fibroblasts. Cells were siRNA treated prior to editing (Fig. 2c) as described in b.
