Supplementary Figure 12: FANCD2 ChIP-seq identifies on- and off-target Cas9 cut sites.
From: CRISPR–Cas9 genome editing in human cells occurs via the Fanconi anemia pathway
a, FANCD2 ChIP-seq overlaps with Guide-seq36 sites. The table describes the top 20 cut sites for the VEGFA_site2 sgRNA or the 10 annotated cut sites for the HEK293_site1 sgRNA. Peaks identified by MACS2 for n = 2 FANCD2 ChIP-seq datasets (green highlighting) are presented along with calculated q values. b, FANCD2 is enriched at the HBB cut site in HEK293T cells. Presented data are ChIP-seq pileups generated from comparing RNP + donor (treatment; n = 2 independent experiments) and Apo (control; n = 2 independent experiments) samples. c, FANCD2 is enriched at VEGFA_site2 on- and off-target loci in HEK293T cells. Presented data are ChIP-qPCR fold enrichments generated from non-specific IgG ChIP and FANCD2 ChIP at the indicated loci. The mean ± s.d. was calculated from n = 2 independent experiments.
