Supplementary Figure 1: SSTR efficiency varies in different human cell lines.
From: CRISPR–Cas9 genome editing in human cells occurs via the Fanconi anemia pathway
Nine cell lines were edited using RNP targeting the EMX1 locus either without or with ssODN containing the PciI sequence. The edited locus was amplified, reannealed, and digested using T7 endonuclease I (t), which quantifies gene disruption, or the restriction enzyme PciI (R), which quantifies SSTR. Data presented are representative of independent experiments.
