Supplementary Figure 2: BFP, GFP, and non-fluorescent populations are effectively resolved during a pooled CRISPRi screen.
From: CRISPR–Cas9 genome editing in human cells occurs via the Fanconi anemia pathway
a, Replicate 1 (of n = 2 independent experiments) of dCas9-KRAB cells infected with gRNA library and edited at the BFP locus. Live single cells were plotted to compare BFP and GFP intensities. Three populations were sorted: BFP+GFP– (BFP), BFP−GFP+ (GFP), and BFP−GFP− (UN). Percentages in each gate are presented for 100,000 cells. b, Total cells sorted for each edited population.
