Supplementary Figure 6: Phenotypic analysis of EBS mutants with disrupted H3K4me3 or H3K27me3 binding.
From: EBS is a bivalent histone reader that regulates floral phase transition in Arabidopsis
a–d, Flowering time analysis of long-day-grown plants from six independent T2 ebs transgenic plants expressing wild-type EBS-FLAG (a), H3K27me3-binding-defective triple-mutant EBS Y49A W70A Y72A (b), H3K4me3-binding-defective mutant EBS Y155A (c), and C-terminus deletion mutant EBSΔC (d). Black horizontal lines are the mean, and error bars represent s.d. from the number of plants (indicated by n) for each line (white dots). e, Western blot analysis of protein expression levels from the plants indicated in a–d using an anti-FLAG antibody. Rubisco serves as a loading control. Two independent lines for each transgene are shown (uncropped images in Supplementary Fig. 8).
