Supplementary Figure 7: Genome-wide H3K4me3 levels in EBS and EBSΔC.
From: EBS is a bivalent histone reader that regulates floral phase transition in Arabidopsis
a,b, Metaplot showing the average levels of EBS and EBSΔC occupancy (a) and H3K4me3 levels (b) over EBS and EBSΔC common target genes in transgenic plants expressing EBS-FLAG and EBS∆C-FLAG. Two independent biological replicates are shown as Rep1 and Rep2. TSS, transcription start site; TTS, transcription termination site. –2 K and + 2 K represent 2 kb upstream of the TSS and 2 kb downstream of the TTS, respectively. The y axis represents read density after normalization with total reads. c, Schematic representation of the workflow for sequential ChIP. d,e, ChIP–qPCR of H3K4me3 (d) or H3K27me3 (e) enrichment relative to input materials in plants expressing EBS-FLAG and EBS∆C-FLAG. Bars denote the mean of two independent experiments.
