Supplementary Figure 6: MLL2 depletion results in impaired developmental gene expression.

a, Representative images of embryoid bodies (EBs) derived from Mll2^WT and Mll2^KO ESCs at day 9 of the differentiation protocol. Images were taken at 100× magnification (scale bar, 400 uM). b, GO term analysis of 2,697 genes significantly upregulated in WT-derived 9-d-old EBs compared to ESCs (day 0). c, GO term analysis of 3,111 genes significantly upregulated in Mll2^KO-derived 9-d-old EBs compared to Mll2^KO ESCs (day 0). d, Venn diagram showing the overlap between the 2,697 genes upregulated in wild-type EBs from day 0 to day 9 and the 3,111 genes upregulated in Mll2^KO EBs from day 0 to day 9. e, GO cellular component analysis of 1,159 genes upregulated only in Mll2^KO EBs from day 0 to day 9. f, MGI mammalian phenotype term analysis of 1,159 genes upregulated only in Mll2^KO EBs from day 0 to day 9.