Supplementary Figure 7: Bisulfite sequencing of NPC differentiation. | Nature Genetics

Supplementary Figure 7: Bisulfite sequencing of NPC differentiation.

From: Gain-of-function DNMT3A mutations cause microcephalic dwarfism and hypermethylation of Polycomb-regulated regions

Supplementary Figure 7

a,b, Locus-specific bisulfite sequencing analysis of Foxa1 (a) and Sox1 (b) of LIF/serum-maintained mESCs. Blocks, independent cell lines; blue and red squares, unmethylated and methylated CpGs, respectively; white squares, undetermined methylation status; columns, CpG sites; rows, individual sequences. Data shown for Foxa1 are representative of n = 2 independent experiments, het cell line n = 1 experiment. Sox1, n = 1 experiment. c, RRBS sequencing confirms DNA methylation gain at DMRs genome-wide upon differentiation. Heatmap of differentially methylated regions (DMRs) after 9-day NPC differentiation. d, Quantification of DNA methylation levels for the hyper-DMRs depicted in c. Datapoints, median percent change in methylation. ∆%mCpG, percent change in methylation relative to the mean for control. P value, two-tailed t test. Horizontal bar, mean per group.

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