Supplementary Figure 15: Further analysis for 50 crosstalk network genes.

a, Individual pathways significantly enriched in the crosstalk. The significance level (FDR), odds ratio (OR) and 95% confidence interval (CI) calculated according to Fisher’s exact test (one-sided), using all multitrait rated genes as the test background. b, Venn diagram illustrating the enrichment of mouse immune-mediated disease phenotypes, using all multitrait rated genes as the test background. Calculated using one-sided Fisher’s exact test. Genes annotated to mouse immune-mediate disease phenotypes sourced from the Monarch Initiative. c, Identification of drug perturbation gene signatures enriched in crosstalk network genes. The significance level (FDR) calculated according to Fisher’s exact test (one-sided). Crosstalk network genes in these enriched signatures shown on the left. PBMCs, peripheral blood mononuclear cells; LPS, lipopolysaccharide; AML, acute myeloid leukemia; CML, chronic myeloid leukemia. d, Heatmap illustrating the potential nodal points/genes and their PDB known protein structures with predicted druggable pockets. Color-coded by the number of druggable pockets found in the structure. e, Multitrait rating versus novelty score, with 41 highly rated but under-explored genes highlighted in box. Also labeled are genes involved in interferon and IL2/6/20 family signaling pathways. f, Illustration of immune system pathways enriched per trait. Trait-specific enrichment based on under-explored genes (identified in e) also rated in top 1% for that specific trait, calculated using one-sided Fisher’s exact test. See Fig. 5a for the layout and coloring.