Supplementary Fig. 5: Characterization of ATAC peaks and gene expression.

a, Volcano plot of differentially accessible chromatin regions (see Methods) for mature NK (left) and monocytes (right). b, PCA of resting (blue) and stimulated (red) ATAC-seq samples including the innate cells (triangles). c, MACS2 peak count across different cell types stratified by condition. MACS2 peak counts were normalized for read count and sample quality effects. The dotted lines indicate the median count of accessible regions in stimulated (red) and resting (blue) samples. d, Heatmap of chromVar TF binding deviation scores (see Methods) for several PWMs (y-axis) and samples (x-axis). e, Gene expression of SPI1 separated by lineage. f, Expression of BATF grouped by lineage and condition. Resting samples indicated in blue and stimulated samples in red. g, Expression of BATF target genes in B cells (left) and Th17 precursors (right). Sample sizes can be found in Supplementary Table 1.