Fig. 4: IFN inducibility of ACE2 and MIRb-ACE2 isoform expression.
a, ACE2 and MIRb-ACE2 isoform expression in NHBE cells following the indicated treatment. b, ACE2 and MIRb-ACE2 isoform expression in Calu-3 cells with or without infection with SARS-CoV-2. c, ACE2 and MIRb-ACE2 isoform expression in the lung of a patient with COVID-19 and in a healthy lung. In a–c, raw data were obtained from study GSE147507. Each bar represents an individual sample. d, Mean (± standard error (s.e.)) ACE2 and MIRb-ACE2 isoform expression, determined by RT–qPCR in SCC-4 and SCC-25 cells with or without IFN stimulation. e, Detection of ACE2 and putative MIRb-ACE2 protein product by western blotting in cell lysates from the same cells as in d. One representative experiment of two is shown. f, Mean (± s.e.) ACE2 and MIRb-ACE2 isoform expression, determined by RT–qPCR in NHBE cells with or without IFN stimulation. In d and f, each symbol represents the mean value of two technical RT–qPCR replicates of a single culture, and the bars and error bars represent the mean and s.e. of the three independently treated cultures in the same experiment.
