Extended Data Fig. 4: Representative IF confocal images of ALI-differentiated primary bronchial epithelial cells stained with 2 commercial anti ACE 2antibodies (green), anti-alpha-tubulin (red), Actin-stain 555 phalloidin (blue) and DAPI (grey). | Nature Genetics

Extended Data Fig. 4: Representative IF confocal images of ALI-differentiated primary bronchial epithelial cells stained with 2 commercial anti ACE 2antibodies (green), anti-alpha-tubulin (red), Actin-stain 555 phalloidin (blue) and DAPI (grey).

From: A novel ACE2 isoform is expressed in human respiratory epithelia and is upregulated in response to interferons and RNA respiratory virus infection

Extended Data Fig. 4

Overlay and separate channels. a, XY imaging plane and XZ and YZ orthogonal confocal slices along white lines using ACE2 C-terminal domain antibody (anti-ACE2 CTD). Scale bar = 20 µm. b, XY imaging plane and XZ and YZ orthogonal confocal slices along white lines using ACE2 ectodomain antibody (anti-ACE2 ECTO). Scale bar = 20 µm. c, Enlarged orthogonal slices using anti-ACE2 CTD showing ACE2 staining along the ciliary axoneme. Overlay and separate channels. Scale bar = 10 µm. d, Enlarged orthogonal slices using anti-ACE2 ECTO showing ACE2 staining along the ciliary axoneme. Overlay and separate channels. Scale bar = 10 µm.

Back to article page