Extended Data Fig. 9: Phenotype analyses of CRISPR/Cas9 mutations for ZmPIF3.3 and TSH4.
From: Genome-wide selection and genetic improvement during modern maize breeding
a, Sequences of ZmPIF3.3 target regions in wild type, Zmpif3.3-2 and Zmpif3.3-3 CRISPR/Cas9 knockout mutants. The target sites and protospacer-adjacent motifs (PAM) are shown as underscored letters and blue letters respectively. The gap lengths of sequences are shown above the wild type sequences. b, Height profile of wild type, Zmpif3.3-2 and Zmpif3.3-3 mutant plants. Bar, 15 cm. c, d, Statistics of plant height (c) and ear height (d) of wild type, Zmpif3.3-2 and Zmpif3.3-3 mutant plants. e, Sequences of TSH4 target regions in wild type and tsh4 CRISPR/Cas9 knockout mutants. f, g, Tassel profile (f) and TBN statistics (g) of wild type and tsh4-1 CRISPR-knockout mutants. Bar, 5 cm. h, i, Tassel profile (h) and TBN statistics (i) of wild type and tsh4-2 CRISPR-knockout mutants. Bar, 5 cm. The p-values of two-tailed t-tests are shown above the plots. Error bars indicate ±s.d.
