Extended Data Fig. 3: Cas9 introduction activates the DNA damage response (related to Fig. 2). | Nature Genetics

Extended Data Fig. 3: Cas9 introduction activates the DNA damage response (related to Fig. 2).

From: Cas9 activates the p53 pathway and selects for p53-inactivating mutations

Extended Data Fig. 3

a, The proportion of cell lines (n = 165) with a positively enriched DNA damage transcriptional signature, following Cas9 introduction. *, P = 0.07; two-tailed Fisher’s exact Test. b, Fluorescent microscopy images of 𝛾H2AX foci (green) and DAPI (blue) in parental TP53-WT HCT116 cells and following Cas9 transduction. Cells with > 5 foci have been marked in white. Scale bar represents 10 µm. c, Quantification of 𝛾H2AX foci from three independent repeats; n = 1,765 and n = 2,523, for WT and Cas9 HCT116 cells, respectively. **, P = 0.0095; one-tailed t test. Data show means, with error bars corresponding to S.D.

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