Extended Data Fig. 5: Comparison between H3K27me3 and non-H3K27me3 FMRs.
a, UCSC genome browser snapshots showing DNA methylation and H3K27me3 enrichment at H3K27me3 (pale blue) and non-H3K27me3 FMRs (light pink). b, Bar charts showing the enriched gene ontology (GO) terms for genes associated with H3K27me3 (top) and non-H3K27me3 FMRs (bottom). P-values (Fisher’s exact test, two-sided, not adjusted by multiple comparisons) are transformed by log10. c, Boxplots showing DNA methylation (left), H3K27me3 enrichment (middle) and nearest gene expression (right) at H3K27me3 and non-H3K27me3 FMRs (n = 2 biologically independent experiments). One-sided P values were calculated by Mann-Whitney U test. The median is indicated by the center lines. The bottom, top edges and whiskers represent the 25th/75th percentiles and 1.5 times of the IQR, respectively. d, Bright field images of cells at day 9 (D9) and day 11 (D11) upon neuron differentiation for WT, TKO, PKO, Eed-/- and PKO;Eed-/- cells (n = 3 biologically independent experiments; one representative image is shown). Red arrows show the cell clones that successfully attach to the plate, and black arrow shows embryonic bodies of PKO;Eed-/- cells that fail to attach to the plate. Scale bar, 50 μm. e, Bar charts showing Pou5f1 expression during differentiation induced by retinoic acid (RA) in various cell lines (n = 2 biologically independent experiments).
