Extended Data Fig. 8: Genotyping of Cas9 deletion clones.
From: Transcription imparts architecture, function and logic to enhancer units
a, Illustration of genotyping PCR amplicon design and size relative to elements targeted for deletion. b, Table listing expected amplicon sizes from various genotypes. ‘-‘ indicates that no amplification is expected. c, Gel images from K562 clonal lines used for qRT-PCR experiments in Fig. 6. (eNMU clones were generated, genotyped and generously provided by the Shendure lab.) Genotyping PCRs were performed only once, but biological replication was achieved through independent clones.
